Killam Seminar Series: Molecular Biology of the Fragile X Syndrome
厂辫别补办别谤:听
Arthur F. Koskinas Professor in Neuroscience, University of Massachusetts Medical School, USA
Registration: available now on
Livestreaming via Vimeo:
础产蝉迟谤补肠迟:听Silencing of FMR1 and loss of its gene product, FMRP, results in fragile X syndrome (FXS). FMRP binds brain mRNAs and inhibits polypeptide elongation. Using ribosome profiling of the hippocampus, we find that ribosome footprint levels in Fmr1-deficient tissue mostly reflect changes in RNA abundance. Profiling over a time course of ribosome runoff in wild-type tissue reveals a wide range of ribosome translocation rates; on many mRNAs, the ribosomes are stalled. Sucrose gradient ultracentrifugation of hippocampal slices after ribosome runoff reveals that FMRP co-sediments with stalled ribosomes, and its loss results in decline of ribosome stalling on specific mRNAs. One such mRNA encodes SETD2, a lysine methyltransferase that catalyzes H3K36me3. Chromatin immunoprecipitation sequencing (ChIP-seq) demonstrates that loss of FMRP alters the deployment of this histone mark. H3K36me3 is associated with alternative pre-RNA processing, which we find occurs in an FMRP-dependent manner on transcripts linked to neural function and autism spectrum disorders.
The Killam Seminar Series at The Neuro
Supported by the generosity of the听, The Neuro鈥檚 Killam Seminar series hosts outstanding guest speakers whose research is of interest to the scientific community at The Neuro and 苹果淫院.
How to participate via Zoom:
1. You can either join a meeting via the invite link or the Meeting ID #.
2. If you click the link it will automatically bring you to the meeting. If you do not have the link and only the meeting ID go to the Zoom application and click 鈥淛oin a meeting鈥� where it will prompt you to input your name and the Meeting ID #.
3. Use the toolbar at the bottom of your screen to mute/unmute yourself and to enable/disable your video.
